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Job Details


The Henry M. Jackson Foundation

Research Associate II - Pathology (RESEA002813)

Healthcare

All

Yearly

No

Bethesda, Maryland, United States

Join the HJF Team!

HJF is seeking a Research Associate II to support the Pathology Department located at the Uniformed Services University in Bethesda, MD. HJF provides scientific, technical, and programmatic support services to Pathology Department.

The Research Associate will have the opportunity to conduct an NCI funded R01 grant focused on the role of Ly6K in TGF-beta and immune escape pathways of triple negative breast cancer and an NCI R21 grant focused on the role of CART cells in solid cancer treatment including pancreatic cancer. Ly6K, a GPI anchored protein, is expressed in cancer tissue and is required for in vivo tumor progression. Upadhyay Lab has shown that Ly6K activates TGF-beta signaling, epithelial-to-mesenchymal transition and PD-L1 expression in cancer cells. Upadhyay Lab discovered pharmacological inhibition of Ly6K by small molecule binders can significantly inhibit multiple distinct aspects of the biological activity of Ly6K. The lab uses a mouse model of cancer involving tumor isograft and xenograft treatment with small molecule, CART and antibody therapeutics administration via IV and IP routes.

The lab values the collaborative environment and has multidisciplinary collaboration fostered to use informatics, molecular dynamics methods in combination with molecular and cellular biology techniques. The Research Associate will have the opportunity to learn new techniques while making seminal contributions to the development of novel immuno-oncology therapeutics for breast and pancreatic cancer.

NOTE: As per Executive Order 14042, all HJF employees are required to be fully vaccinated against COVID-19. Proof of vaccination or an approved religious or medical accommodation will be required.

Responsibilities:

  1. Performs routine data analysis and basic interpretation and verifies and corrects data entry. (20%)
  2. Trains junior laboratory staff in laboratory techniques and processes, as needed. (10%)
  3. Conducts research in collaboration with other scientific and technical staff. (5%)
  4. Performs complex laboratory tests, determinations, and analyses involving multiple procedures and a variety of technical approaches. (50%)
  5. Documents current or new procedures or protocols for sample selection, data collection, data processing, and/or statistical estimation. (10%)
  6. Analyzes and prepares reagent and/or assay product documentation. Organizes and procures needed reagents, performs experiments, analyzes data, and documents results. (5%)
  7. May perform other duties and responsibilities as assigned or directed by the supervisor. This may include attendance of and participation in required training for role.

Required Knowledge, Skills and Abilities:

  1. Proficient in working with mice model of cancer such as subcutaneous injection in to mammary gland, tumor measurement, intravenous and intraperitoneal injections.
  2. Proficient in protein estimations, SDS-PAGE gel run, western blotting, western blot quantification using image J.
  3. Proficient in RNA isolation, qRT PCR setup using quantstudio 6 instrument.
  4. Proficient in isolating genomic DNA using Qiagen kit and perform genomic PCR.
  5. Proficient in cell culture of breast, pancreatic cell lines.
  6. Experience in immunofluorescence, immunohistochemistry.
  7. Experience in using confocal microscope.
  8. Familiarity with Flow cytometry.
  9. Ability to work alone or in the team as needed.
  10. Ability to meet deadlines.

Minimum Education: Master’s degree required.

Minimum Experience/ Training Requirements: Minimum 3-5 years of experience required.

Physical Capabilities: Position requires long periods of standing.

Work Environment: This position takes place in a laboratory setting.

Work Arrangement: 100% in-person.

Background/Security: Eligibility to obtain and maintain a Tier I investigation/Public Trust.

Employment with HJF is contingent upon successful completion of a background check, which may include, but is not limited to, contacting your professional references, verification of previous employment, addresses, education, and credentials, a criminal background check, drug screening, and a department of motor vehicle (DMV) check.

Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Chief Human Resources Officer.