Postdoctoral Fellow (Cancer Research) – Pathology
Join the HJF Team!
HJF is seeking a Postdoctoral Fellow to support the Pathology located at Uniformed Services University in Bethesda, Md. HJF provides scientific, technical, and programmatic support services to Pathology.
The Postdoctoral Fellow will have the opportunity to conduct research focused on the role of Ly6K in TGF-beta and immune escape pathways of triple negative breast cancer as well as research focused on the role of CART cells in solid cancer treatment including pancreatic cancer.
Ly6K, a GPI anchored protein, is expressed in cancer tissue and is required for in vivo tumor progression. Dr. Upadhyay Laboratory has shown that Ly6K activates TGF-beta signaling, epithelial-to-mesenchymal transition and PD-L1 expression in cancer cells. Dr. Upadhyay Laboratory discovered pharmacological inhibition of Ly6K by small molecule binders can significantly inhibit multiple distinct aspects of the biological activity of Ly6K. The laboratory uses a mouse model of cancer involving tumor isograft and xenograft treatment with small molecule, CART and antibody therapeutics administration via IV and IP routes.
The laboratory values the collaborative environment and has multidisciplinary collaboration fostered to use informatics, molecular dynamics methods in combination with molecular and cellular biology techniques. The incumbent will have the opportunity to learn new techniques while making seminal contributions to the development of novel immuno-oncology therapeutics for breast and pancreatic cancer.
NOTE: As per Executive Order 14042, all HJF employees are required to be fully vaccinated against COVID-19. Proof of vaccination or an approved religious or medical accommodation will be required.
- Assists in designing, developing, executing, and implementing scientific research and/or development. (60%)
- Investigates the feasibility of applying a wide variety of scientific principles and theories to potential inventions and products. (5%)
- Performs specialized laboratory research utilizing experimental protocols which involves specialized procedures. (10%)
- Collects and handles samples and keeps detailed records of experiments. (10%)
- Assists with the preparation of data for presentations at scientific meetings and for publication in journals. (5%)
- Performs specialized laboratory research utilizing experimental protocols which will involve specialized procedures such as DNA cloning and sequencing, Southern blotting, molecular analysis of specific RNA transcripts by quantitative RT-PCR or Northern analysis, animal injections, bleedings and immunizations.
- May perform other duties and responsibilities as assigned or directed by the supervisor. This may include attendance of and participation in required training for role.
- Assists with training laboratory staff as needed.
Required Knowledge, Skills and Abilities:
- Knowledge of appropriate scientific area; ability to analyze and interpret data.
- Knowledge of Flow cytometry: Sample preparation, execution on the machine, data analysis, sorting and multicolor panel.
- Cell culture: Primary and cell lines, T cell expansion.
- Protein estimation.
- In vivo studies: Mouse handling, i.p. and i.v. injections, tumor injections and measurements, immunotherapy assays, tissue collection and processing, mouse genetics.
- In vitro and ex vivo assays: cell signaling, live cell imaging, westerns, qRT-PCR, luminex.
Minimum Education: Doctoral degree required.
Minimum Experience/ Training Requirements: A minimum of 0-5 years of experience required.
Work Environment: This position takes place in a laboratory setting.
Work Arrangement: 100% on-site.
Background/Security: Eligibility to obtain and maintain a Tier I investigation/Public Trust and a Common Access Card (CAC).
Employment with HJF is contingent upon successful completion of a background check, which may include, but is not limited to, contacting your professional references, verification of previous employment, addresses, education, and credentials, a criminal background check, drug screening, and a department of motor vehicle (DMV) check.
Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Chief Human Resources Officer.